Optimization of a freeze-drying cycle of a viral vaccine based on changes in temperature, time and geometry of the vials.

The freeze-drying process is used in pharmaceutical industry, however in terms of process management, the process must be avoided at all cost because it has several disadvantages such as high equipment investment, high energy demand, a process that requires long times and products easily to moisturize and fragile and needs to be carefully packed and stored. This work aims at reducing the freeze-drying cycle time of a viral product and consider loading this product at subzero temperatures to increase productivity of this product. Experiments were carried out with freeze-drying cycles with less 15 and 20h in comparison to the original cycle. The experiments were performed with the same formulation of the commercial batch and using a pilot freeze dryer. Modifications were made in the physical properties of the current freeze-drying cycle (temperature, pressure and time) and the loading temperature of the product, without changing the formulation of the vaccine or primary loading. Samples of the lyophilized product trial were analyzed for their appearance, performance, residual moisture, strength and accelerated thermostability in the amount currently used by the company quality control. All the results were within the specifications of the company were close to or better when compared to commercial batches.

Similar Sealability Between Bioceramic Putty Ready-To-Use Repair Cement and White MTA

This study was designed to assess the ability to prevent glucose penetration of a bioceramic putty ready-to-use repair cement comparing to white mineral trioxide aggregate (WMTA). After root canal instrumentation, the apical 3 mm of maxillary incisors were resected and root-end cavities with depth of 3 mm were prepared with ultrasound and filled with the tested materials (15 roots per group). All roots were mounted in a double chamber system to assess glucose penetration using 15 psi pressure application. After 1 h, glucose concentrations in the lower chamber were measured following an enzymatic reaction. Four roots were used as controls. The Mann-Whitney test verified differences in glucose leakage between groups and the Tukey's test was used for multiple comparisons. Significance level was set at 5%. There was no significant difference in glucose leakage between iRoot BP Plus and White MTA groups. iRoot BP Plus had a similar ability to that of white MTA in preventing glucose leakage as a root-end filling material.

Este estudo foi desenhado para avaliar a capacidade de impedir a infiltração de glicose de um cimento reparador biocerâmico pronto para o uso, em comparação com o agregado trióxido mineral (MTA) branco. Após a instrumentação do canal radicular, os 3 mm apicais de incisivos superiores foram removidos, e retropreparos com 3 mm de profundidade foram realizados com ultra-som. As cavidades retrógradas foram preenchidas com os materiais testados (15 raízes por grupo). Todas as raízes foram montadas em um sistema de câmara dupla para avaliar a infiltração de glicose utilizando a aplicação de pressão de 15 psi. Depois de 1 h, concentrações de glicose na câmara inferior foram medidas seguida de uma reação enzimática. Quatro raízes foram usadas como controle. O teste de Mann-Whitney verificou diferenças na infiltração de glicose entre os grupos e o teste de Tukey realizou comparações múltiplas. A significância foi estabelecida em α= 5%. Não houve diferença significante entre a média e a mediana comparando-se os grupos do iRoot BP Plus e o MTA branco. iRoot BP Plus teve uma capacidade semelhante ao do MTA branco na prevenção da infiltração de glicose como material retroobturador.

Batch and fixed-bed column biosorption of manganese ion by sargassum filipendula

This paper presents experimental data of the biosorption of manganese onto Sargassum filipendula in both batch and fixed-bed column systems. Batch equilibrium data were used for nonlinear fittings of the Langmuir and Freundlich isotherms. A mathematical model based on mass balances in the fluid and in the sorbent was applied to represent the experimental fixed-bed column data. The utilization of isotherm parameters from the batch experiments in the breakthrough model implied a significant mismatch in relation to the laboratory data. Alternatively, additional fixed-bed column data provided new parameters for the isotherm evaluation, and the corresponding simulated profile of the breakthrough curve reached better agreement to the experimental results.